Effect of Pore Structure on Cell Proliferation in Bio-printed Scaffold

Abstract

In tissue engineering, scaffolds play a role in maintaining the three-dimensional shape of tissue when culturing cells. The scaffold should have a porous structure. The rate of cell adhesion and proliferation varies depending on the pore shape and size. The factor that can determine the pore shape are the angle between struts that make up the scaffold. In this study, cell adhesion and proliferation are analyzed with the strut angle. Three types of scaffold with 30 , 60 , 90 respectively, are manufactured using bio printing. Cell adhesion is interpreted as sedimentation by gravity. Cell proliferation is interpreted as surface tension due to difference of adhesion force of cell-cell and cell-scaffold. By measuring the curvature of cell proliferation due to surface tension, the ratio of the pressure difference between the cell and the media and the surface tension can be known. The tendency of this ratio can be expressed in terms of time and strut angle. In addition, the curvature can be used to calculate the volume of cell proliferation. The tendency of volume of cell proliferation can be expressed in terms of time and strut angle. Based on these study, the cell proliferation can be predicted in the designed scaffold. It is also possible to control cell proliferation through the structure of the scaffold when making the tissue.