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NRXN1 as a Prognostic Biomarker: Linking Copy Number Variation to EMT and Survival in Colon Cancer

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Abstract
The role of biomarkers in cancer treatment varies significantly depending on the cancer stage. Thus, in clinical practice, tailoring biomarkers to meet the specific needs and challenges of each cancer stage can increase the precision of treatment. Because they reflect underlying genetic alterations that influence cancer progression, copy number variation (CNV) biomarkers can play crucial prognostic roles. In our previous study, we identified potential survival-related genes for colorectal cancer (CRC) by analyzing CNV and gene expression data using a machine-learning approach. To further investigate the biological function of NRXN1, we assessed the use of RNA sequencing, phosphokinase assays, real-time quantitative PCR, and Western blot analysis. We found that NRXN1 copy number deletion was significantly associated with poor overall survival (OS) and recurrence-free survival (RFS), even in patients who received adjuvant chemotherapy. Compared with its expression in normal tissues, NRXN1 expression was lower in tumors, suggesting its potential role as a tumor suppressor. NRXN1 knockdown enhanced CRC cell viability and invasion, and transcriptome analysis indicated that the increased invasion was caused by GSK3 beta-mediated epithelial-mesenchymal transition. These findings highlight NRXN1 copy number deletion as a novel biomarker for predicting recurrence and survival in patients with resected colon cancer.
Author(s)
Bang, Hyun JinShim, Hyun-JeongPark, Mi-RaYoon, SuminYoo, Kyung HyunKim, Young-KookLee, HyunjuNam, Jeong SeokHwang, Jun-EulBae, Woo-KyunChung, Ik-JooSun, Eun-GeneCho, Sang-Hee
Issued Date
2024-11
Type
Article
DOI
10.3390/ijms252111423
URI
https://scholar.gist.ac.kr/handle/local/8575
Publisher
MDPI
Citation
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.25, no.21
ISSN
1661-6596
Appears in Collections:
Department of AI Convergence > 1. Journal Articles
Department of Life Sciences > 1. Journal Articles
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