Ginsenoside-Re-rich ethanol extract of Panax ginseng berry enhances healthspan extension via mitostasis and NAD metabolism

Abstract

Background Ginseng Berry Concentrate (GBC) enhances exercise capacity in mice, but the effects of its key component, ginsenoside Re (G-Re), on aging and mitochondrial function are not well understood. This study investigates the impact of G-Re on mitophagy and its potential to promote healthy aging. Methods Experiments in C2C12 myocytes and HeLa-mitoKeima-PARKIN cells assessed GBC and G-Re's effects on mitophagy, supported by Gene Set Enrichment Analysis. G-Re was identified as the primary component of GBC via high-performance liquid chromatography. The influence of G-Re on lifespan and healthspan was examined in Caenorhabditis elegans, with a focus on mitophagy pathways. Results GBC and G-Re significantly induced mitophagy and enhanced mitochondrial gene expression, improving mitochondrial function. G-Re extended lifespan and healthspan in C. elegans, effects absent in mitophagy-impaired mutants. Conclusion G-Re enhances mitochondrial function and promotes healthy aging through mitophagy, suggesting its potential for mitigating age-related functional declines.