Oncogenesis-specific RNA alternative splicing regulation (암 특이적 RNA alternative splicing 조절 연구)

Abstract

RNA alternative splicing (AS) is a posttranscriptional process to form various mRNA isoforms from one pre-mRNA. Aberrant RNA alternative splicing is frequently found in the development of breast cancer. RNA-mediated oligonucleotide Annealing, Selection, and Ligation with Next-Generation sequencing (RASL-seq) has been performed previously to compare alternative splicing between human breast cancer (MCF7) and normal breast (MCF10A) cells. I performed Gene Ontology (GO) analysis for the genes within increased or decreased cassette exons in human breast cancer cells. I identified that numerous oncogenesis related functions are enriched in these genes. I further validated these events using RT-PCR analysis and found that 10 of increased and 10 of decreased splicing events of cassette exons were similar to RASL-seq results, indicating a high validation rate of RASL-seq. While the mRNA isoforms increased in human cancer cells are breast cancer specific isoforms. Therefore, I discovered oncogenic alternative splicing events that represent potential targets for therapeutic development.