Quantitative lipidome analysis of serum from mouse exposed to microplastic using deuterium oxide labeling

Abstract

Microplastics(MPs) are small (typically less than 5 mm) pieces of plastic fragments and have become global environmental and health concerns due to pollution of the aquatic system including oceans, rivers, and lakes as well as air and soil. To study the in vivo effects of low concentrations/long exposure time of MPs on mammals, untargeted lipidomic analysis was performed with a mouse model based on Deuterium Oxide (D2O) Labeling for Global Omics Relative Quantification (DOLGOReQ) technique that I have recently developed. For the experimental group, ICR mice were administrated with MPs in drinking water for 5 and 24 weeks. There were 144 to 172 common lipidomes in the experimental group. In the relative quantitative analyses, triacylglycerol, lysophosphatidylcholine, and sphingomyelin showed significant changes over 5 weeks and 24 weeks. I identified metabolism involving the changed lipid class, and noted probable issue about glycerolipid, glycerophospholipid, and sphingolipid metabolism.