Colorimetric detection of glucose based on the enhanced peroxidase-like activity of [Mn2(bpmp)]3+ by ADP

Abstract

Enzyme-coupled reactions of horseradish peroxidase (HRP) and various other oxidases have been intensively used in the field of biosensing. However, the intrinsic drawbacks of HRP, including sensitivity to assay conditions, high cost for preparation, purification, and storage processes, limit further applications of HRP in biosensing. To overcome these drawbacks, we herein designed colorimetric assay for glucose based on [Mn2(bpmp)]3+, which possesses peroxidase-like activity and whose activity was notably enhanced by ADP. Under [Mn2(bpmp)]3+/ADP/ABTS/glucose oxidase system, real-time detection of glucose was successfully performed at 25 ℃ and pH 7.0 in one step sequence. As an extension, colorimetric detection of cholesterol was also conducted based on our system.