Stabilization of immune synapse using recombinant Transgelin 2_PTD protein and adoptive T cell therapy for cancer.

Abstract

Actin has critical roles in the formation of immune synapse and the effector function in cytotoxic T cells. However, research of the mechanism of regulating actin is still unclear. We stabilized actin cytoskeleton using Transgelin 2, an actin binding protein reported in previous studies, to increase theeffect of cytotoxic T cells.In addition, we found that OVA-E0771 cells expressing ICAM-1 were inhibited by increasing the cytotoxic effect of OT1 CD8+ T cells on the basis that Transgelin 2 regulated actin by physically associated with LFA-1. However, OVA-B16F10 cells lacking ICAM-1 could not show the effect. We used recombinant Transgelin 2 with Protein transduction domain (PTD) to complementthe problems of viral transduction while applying the effects of Transgelin 2 to cancer therapy. The activation of actin-LFA-1 axis through recombinant Transgelin 2, can be presented as an effective method for adoptive T cell immunotherapy.