Development of an aptasensors based on localized surface plasmon resonance using a new Saxitoxin aptamer

Abstract

Seafood is emerging as a health food, and interest in improving the quality of seafood is increasing. Saxitoxin(STX) is a neurotoxin, which is produced by marine dinoflagellates and accumulated in seafood. It can block the neuronal transmission between nerves and muscle cell membranes, resulting in subsequent disturbance of neuromuscular transmission and voluntary muscle paralysis. Here, we developed new aptamer for detection of STX by use of graphene oxide– the systematic evolution of ligands by exponential enrichment (GO-SELEX). Furthermore, we confirmed the ability of sensitivity and selectivity of the developed aptamer to STX using localized surface plasmon resonance (LSPR) sensor. The LSPR aptasensor showed good responses in a broad dynamic range from 5 to 10,000 ppb with a good linearity (R2=0.9896), and a limit of detection (LOD) is reacted to 2.41 ppb. Moreover, STX in real mussel samples could be successfully determined with good recoveries (93.88% - 118.61%). The results demonstrate that the new STX aptamer-modified GNR chip has an applicability to be utilized in real filed for detection of STX in real samples with accurate sensing performances.