전기중합 방법을 이용한 분자 각인 고분자 (Molecularly imprinted polymer)기반의 식중독 감염균 검출 센서 개발

Abstract

The molecularly imprinted polymer (MIP) refers to a matrix polymer synthesized by covalent or non-covalent interaction with a biological analyte, which provides a template for a selective recognition site for the analyte. Since most MIPs are hydrophobic, there is a limitation in that they have a weak recognition ability to provide a biological recognition template in an aqueous solution. Electropolymerization forms a MIP by covalent interaction between the analyte and conductive polymer using cyclic voltammetry. It has advantages of strong affinity and selectivity for biorecognition, excellent physical and chemical stability. In study, the conductive polymer with a high affinity for Vibrio. parahaemolyticus (VP), known as a food-born pathogen, was selected by a molecular modeling simulation. It is used to form a MIP upon a gold nanostructured electrode using electropolymerization. Then, we conducted the electrochemical signal analysis according to the synthesis mechanism of MIP and the signal change with the VP