금 나노 구조 전극 기반의 전기중합 분자 각인 고분자를 이용한 흰반점 바이러스 검출 센서 개발

Abstract

The electropolymerized molecularly imprint polymers (EMIPs) are thin films with a particular molecular recognition site for a template molecule by an external current. It is resistant to high temperatures and pressure and has excellent stability against acids, bases, and organic solvents. In addition, it has excellent reproducibility and adhesion to the electrode surface; furthermore, it is characterized by low time and cost consumption in the synthesis optimization process. By applying these advantages, this study aims to develop an electrochemical sensor that detects the WSSV (white spot syndrome virus), classified as an aquatic organism type 1 infectious disease. A ortho-phenylenediamine (o-PD) monomer with the highest affinity for the WSSV outer membrane protein (VP28), a template molecule, was selected using an AutoDock molecular modeling simulation. Subsequently, the electropolymerization mechanism on the gold nanostructured electrode (GNE) was identified as a cyclic voltammogram. It confirmed that the water-soluble monomer was synthesized into a polymer through a redox reaction. Lastly, the electrochemical signal difference was analyzed following each step.