Quantitative Proteomic Analysis of Human Neutroblastoma Cells Exposed to Particulate Matter

Abstract

Particulate matter (PM) is known to be an environmental risk factor that gives rise to deleterious effects on human health. Owing to its chemical components (e.g., polycyclic aromatic hydrocarbons, NO2, SO2, and so on), inhalation of PM might cause diverse diseases, such as, cardiovascular, respiratory, eye, and skin diseases. Until now, PM studies have generally been focused on the circulatory and respiratory systems. In this study, an effect of PM on the nervous system was investigated using PM exposed neuroblastoma cell (SH-SY5Y). Proteins obtained from PM exposed SH-SY5Y were compared with those from normal SH-SY5Y to obtain biomarker candidates. A global quantitative proteomic analysis of two SH-SY5Y samples was performed using isobaric tags for relative and absolute quantification (iTRAQ) based 2D nano-liquid chromatography-electrospray ionization-tandem mass spectrometry. We confirmed that proteins related to neural development were reduced and those related to cell death were increased due to PM exposure.