Molecularly imprinted electrochemical sensor based on the poly(o-phenylendiamine-co-o-aminophenol) for detection of White Spot Syndrome Virus antigen.

Abstract

This study proposes the molecularly imprinted polymer (MIP) based electrochemical sensor for detecting the VP28 of white spot syndrome virus (WSSV) antigen. The MIPs provide a specific recognition site and can interact with target molecules like an antibody. It was electropolymerized with a mixture of functional monomers around an immobilized virus antigen on the electrode surface. The molecular simulation chose the o-PD and o-AP monomers with the highest affinity for the VP28. These monomers can be synthesized to copolymer using cyclic voltammetry with a repeated redox mechanism. The MIP-based electrochemical sensor indicates reasonable changing DPV plots to prove the detection of VP28. The fabricated copolymer was hindered in the electron transfer of the redox probe by binding the VP28. So, it displays the proportionally increased calibration curve of the DPV response versus the concentration of VP28. In the future, we aim to use the real sample and expect this sensor to be applied as a pre-diagnostic method for WSSV detection.