Systematic modulation of multi-enzymatic activities of metalloporphyrin-conjugated heparin ROS scavenging

Abstract

This paper presents the development of a heparin micelle-histidine system aimed at modulating the ratio of antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT). Metalloporphyrins (Fe, Mn, Cu, Zn)-conjugated heparin particles were successfully synthesized and characterized. The incorporation of histidine 10-mer (His10) affected the surface charge, size, and polydispersity index (PDI) of the heparin-porphyrin complexes with the effects varying depending on the metal ions in metalloporphyrins. In addition, the activities of SOD, CAT, and POD enzymes were significantly influenced by the addition of His10, and this effect was metal-dependent for each micelle. Mixed micelles allowed for precise control of SOD and CAT activities. In vitro experiments revealed that the HFe-H10 (1:2) micelles, which exhibited the highest SOD and CAT activities among the tested micelles, exhibited enhanced cellular protective effects against superoxide anions and hydrogen peroxide. Overall, this study provides valuable insights into the application of the heparin micelle-His10 system as a modulator of enzymatic activity in biomedical settings.