High-resolution Laser Speckle Microscope with Common Axial Illumination
- Abstract
- ABSTRACT
Laser Speckle Contrast Imaging (LSCI) is a simple, label-free, and non-invasive optical imaging technique, capable of acquiring images with a high spatiotemporal resolution. The measurement strategy is based on quantification of fluctuations in a speckle pattern to monitor the movement of particles. In this study, modified from the previously well-known oblique illumination, a microscopic speckle imaging device was designed based on epi-illmination through the object lens. Specifically, we hypothesized that illuminating the sample surface with either linearly or circularly polarized light at a specific angle, which is evaluated by the analyzer would enhance the speckle contrast. As a result, a significantly enhanced image contrast by linear polarization could be achieved with the optimization of imaging parameters, which was sufficient for assessing the presence of flow in microvascular structure. For circularly polarized light, we determined the phase by passing it through the quarter-wave plate twice, in which the phase was controlled for the contrast optimization When compared with the linear polarization results, the image contrasts obtained by varying phase of the circularly polarized light was not deterministic, thus could not be optimized. As a conclusion of the study, via addition of microscopic imaging capability and investigation of the effects of excitation light polarization, we designed a laser speckle microscope system to improve the speckle imaging contrast and also resolution by optimizing laser speckle size. Such advances would greatly increase the utility of laser speckle for assessing microscopic movements in a biological structure with enhanced efficiency.
- Author(s)
- Jeongmyo Im
- Issued Date
- 2023
- Type
- Thesis
- URI
- https://scholar.gist.ac.kr/handle/local/19342
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