Exploring the physiochemical properties and bio-usage of upconverting nanoparticles

Abstract

Lanthanide-doped upconverting nanoparticles (UCNPs) are extremely recognized as a potential apparatus for biological imaging, fundamentally assigned to their unique optical features. These nanoparticles show raised photostability and minimum cytotoxicity inside cellular environment. Lanthanide-doped upconverting nanoparticles release visible photons by the upconversion process after the absorption of two or more near-infrared (NIR) photons, allowing for two-color imaging for cellular activities. In the area of cellular imaging, the application of upconversion nanoparticles has unlocked exciting paths for investigating cellular operations with increased clarity and minimum intrusiveness. This study brings into focus the usage of Erbium (Er3+) and Thulium (Tm3+) doped NaYF4 upconverting nanoparticles for dual color live-cell imaging of HeLa cells. The side-by-side existence of Er3+ and Tm3+ dopants inside the NaYF4 lattice structure permits for synchronized envision of different cellular activities with clear fluorescence emissions presenting a flexible platform for studying dynamic processes in HeLa cells. This research depicts an outstanding step in proceeding the effectiveness of live cell imaging methods and grips promise for an intense understanding of cellular biology.