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Effect of adhesion force-induced trogocytic molting on T cell in immune response

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Abstract
Trogocytic molting, a process involving the shedding of microvilli-derived immunological synaptosomes (TIS), plays a critical role in T cell activation and immune synapse formation. This study demonstrates that during immune synapse maturation and the subsequent kinapse phase, T cells release TIS enriched with T cell receptors (TCRs). We identified that adhesion forces, primarily mediated by LFA-1, drive this molting process, facilitating TCR-dependent T cell proliferation. Moreover, trogocytic molting significantly enhances T cell activation by activating the PI3K-AKT-mTOR signaling pathway and boosting cellular metabolic activity. These findings reveal trogocytic molting as a crucial mechanism for efficient immune responses, linking adhesion dynamics to metabolic reprogramming in T cells.
Author(s)
김민상
Issued Date
2025
Type
Thesis
URI
https://scholar.gist.ac.kr/handle/local/19212
Alternative Author(s)
Min Sang Kim
Department
대학원 생명과학부
Advisor
Jun, Chang-Duk
Table Of Contents
ABSTRACT i
CONTENTS ii
LIST OF FIGURES iv
1. Introduction 1
2. Materials and methods 4
2.1. Reagents and antibodies 4
2.2. Cells 4
2.3. Animals 5
2.4. T cell activation 5
2.6. Western blot analysis 6
2.7. Confocal microscopy 6
2.8. Electron microscopy 7
2.9. Flow cytometry analysis 8
2.10. Seahorse Mito Stress assay 9
2.11. Statistics 9
3. Results 10
3.1. Actin remodeling and microvilli dynamics drive the formation and release of
immunological synaptosomes during T cell activation 10
3.2. Adhesion forces drive trogocytic molting and surface TCR downregulation to
enable proliferation of T cells 11
3.3. Trogocytic molting enhances PI3K-AKT-mTOR signaling, immune synapse formation,
and metabolic activity in T Cells 13
4. Discussion. 20
5. Abstract in Korean (국문요약) 22
6. References 23
Degree
Master
Appears in Collections:
Department of Life Sciences > 3. Theses(Master)
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