A PCR-LFA Paper Chip for the Naked-Eye Detection of Campylobacter jejuni

Abstract

Molecular diagnostics are pivotal for the rapid and accurate detection of pathogens. However, their traditional implementation often requires complex equipment and skilled personnel, limiting their utility in resource-limited and point-of-care (POC) settings. To address these limitations, paper-based point-of-care testing (POCT) platforms have emerged as a promising solution, offering low costs, portability, and ease of use for rapid diagnostics in constrained environments. In this study, we developed a paper-based platform that integrates a paper membrane-based polymerase chain reaction (PCR) with a lateral flow assay (LFA), enabling sensitive and visual nucleic acid detection. The platform employs gold nanoparticles (AuNPs) conjugated with sequence-specific complementary probes to detect amplified PCR products visually. Using this system, we successfully detected Campylobacter jejuni at concentrations as low as 1 copy µl-1 within 30 minutes. This platform combines the high sensitivity and specificity of PCR with the simplicity and affordability of paper-based diagnostics. Its rapid detection capabilities, minimal equipment requirements, and user-friendly design make it a practical solution for nucleic acid detection in POC settings. This system holds significant potential for clinical diagnostics, environmental monitoring, and other applications in resource-limited environments.