Advancing Quantitative Phase Imaging for Biological Studies using Single-Shot Transport-of-Intensity Equation and Beam Splitting Assemblies

Abstract

Quantitative phase imaging is a technique to extract the phase information of the transparent sample being investigated. One quantitative phase imaging technique is the transport of intensity equation (TIE), which is a non-interferometric method that relates the intensities as a function of position with the phase. Current trends in this technique are by doing single-shot, which does not require any mechanical translation. Thus, improving its efficiency in capturing images. In these studies, a wollaston prism was used to split the beams with optical glass plate placed on one beam to induce a controlled defocus. On the second part of the study, the technique is implemented using a lateral displacement beam splitter. This technique allows quantitative phase imaging with the advantage of a single-shot measurement