Crystallization and preliminary crystallographic studies of ribosome recycling factor from Escherichia coli

Abstract

Ribosome recycling factor (RRF) catalyzes the disassembly of a termination complex during the final stage of protein synthesis. RRF from Escherichia coli has been crystallized with PEG 400 as precipitant at 287 K. The crystal belongs to the trigonal space group P3(1)21 (or P3(2)21), with unit-cell parameters a = b = 48.08, c = 141.67 Angstrom. Native data were collected from a frozen crystal to a resolution of 3.0 Angstrom on a Cu K alpha rotating-anode X-ray source.