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Crystallization and preliminary X-ray crystallographic studies of response regulator for cyanobacterial phytochrome, Rcp1

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Author(s)
Im, YJ; Park, CM; Kim, JI; Yang, SS; Kang, JG; Rho, SH; Kim, Jae Il; Song, Woo Keun; Song, PS; Eom, Soo Hyun
Type
Article
Citation
Acta Crystallographica Section D: Biological Crystallography, v.56, no.11, pp.1446 - 1448
Issued Date
2000-11
Abstract
The key response-regulator gene of light regulation, rcp1, from Synechocystis sp. has been overexpressed, purified and subsequently crystallized using ammonium sulfate as a precipitant in forms suitable for X-ray crystallographic studies. A native data set was collected to a resolution of 2.5 Angstrom at cryogenic temperature. The crystals belong to the hexagonal space group P6(3), with unit-cell parameters a = b = 89.04 (5), c = 60.29 (3) Angstrom. The Matthews parameter suggests that Rcp1 crystallizes with two molecules per asymmetric unit.
Publisher
MUNKSGAARD INT PUBL LTD
ISSN
0907-4449
DOI
10.1107/S0907444900009951
URI
https://scholar.gist.ac.kr/handle/local/18607
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