Microbial removal of uranium in uranium-bearing black shale

Abstract

The effects of several conditional factors on efficiency of U bioleaching using an iron-oxidizer, Acidithiobacillus ferrooxidans, from U-bearing black shale (349mg kg(-1) of U) were investigated. When batch-type reactors containing black shale were initially inoculated with the cells, lower pH, higher redox potential and higher amount of aqueous Fe3+ than those of non-inoculated reactor were observed until 200h. Such development of condition, which was facilitated by microbial activity, can enhance the rate and extent of U leaching from the solid substrate. However, under the condition of enough nutrients and energy source (Fe2+) supplied, indigenous Fe-oxidizers in the non-inoculated black shale were activated over time. They exerted almost same influence on the leaching efficiency with the inoculated samples after 250h. Low initial Fe2+ supply (5g l(-1)) and no addition of inorganic nutrients resulted in nearly identical extent of U leaching with that of 9g l(-1) of initial Fe2+ and nutrients supply. The results indicate that, in a practical process of bioleaching, the expenses for Fe2+ and nutrients addition can be reduced. (c) 2004 Elsevier Ltd. All rights reserved.