Crystallization and preliminary X‐ray crystallographic analysis of the GluR0 ligand‐binding core from Nostoc punctiforme

Abstract

GluR0 from Nostoc punctiforme (NpGluR0) is a bacterial homologue of the ionotropic glutamate receptor. The ligand-binding core of NpGluR0 was crystallized at 294 K using the hanging- drop vapour-diffusion method. The l-glutamate-complexed crystal belongs to space group C222(1), with unit-cell parameters a = 78.0, b = 145.1, c = 132.1 angstrom. The crystals contain three subunits in the asymmetric unit, with a V-M value of 2.49 angstrom(3) Da(-1). The diffraction limit of the L-glutamate complex data set was 2.1 angstrom using synchrotron X-ray radiation at beamline BL-4A of the Pohang Accelerator Laboratory (Pohang, Korea).