GIST Scholar
College of Life Sciences and Medical Engineering
Department of Life Sciences
1. Journal Articles
Crystallization and preliminary X-ray crystallographic analysis of PAS factor from Vibrio vulnificus
- Type
- Article
- Citation
- Molecules and Cells, v.20, no.3, pp.361 - 363
- Issued Date
- 2005-12
- Abstract
- Plasmid Achromobacter secretion (PAS) factor is a putative secretion factor that induces the secretion of periplasmic proteins. PAS factor from Vibrio vulnificus was crystallized at 294 K by the hanging drop vapor-diffusion method. It was isolated as a monomer during the purification procedures. The native crystal belongs to the F222 space group with unit cell parameters a=56.1, b=74.4, c=80.0 angstrom, alpha=beta=gamma=90 degrees. The crystal was soaked in cryoprotectant containing 1 M NaBr for 1 h for MAD phasing. The diffraction limit of the Br-MAD data set was 1.9 angstrom using synchrotron X-ray irradiation at beam line BL-18B at the Photon Factory, Japan.
- Publisher
- 한국분자세포생물학회
- ISSN
- 1016-8478
- Appears in Collections:
- Department of Life Sciences > 1. Journal Articles
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