Crystallization and preliminary X-ray crystallographic analysis of PAS factor from Vibrio vulnificus

Abstract

Plasmid Achromobacter secretion (PAS) factor is a putative secretion factor that induces the secretion of periplasmic proteins. PAS factor from Vibrio vulnificus was crystallized at 294 K by the hanging drop vapor-diffusion method. It was isolated as a monomer during the purification procedures. The native crystal belongs to the F222 space group with unit cell parameters a=56.1, b=74.4, c=80.0 angstrom, alpha=beta=gamma=90 degrees. The crystal was soaked in cryoprotectant containing 1 M NaBr for 1 h for MAD phasing. The diffraction limit of the Br-MAD data set was 1.9 angstrom using synchrotron X-ray irradiation at beam line BL-18B at the Photon Factory, Japan.