Combined toxicity of copper and phenol derivatives to Daphnia magna: Effect of complexation reaction

Abstract

The complexation of Cu with phenol was investigated in aqueous solution to find the changes in toxicity toward Daphnia magna in mixtures of copper and phenol derivatives and determine an appropriate prediction model for the toxicity of these mixtures. In the titration experiment, the results showed that phenol played all important role in the remarkable reduction of the Cu2+ concentration, due to its complexation with Cu, with the subsequent reduction of the toxicity of aqueous mixtures containing both Cu and phenol. As a result, it was clearly demonstrated that Cu-phenol formed a non-toxic complex toward D. magna as the mortality declined, despite the addition of phenol to a fixed Cu concentraion. Meanwhile, prediction of the combined toxicity tor binary mixtures of Cu and II phenol derivatives more accurately followed an independent action model (p = 0.143, df = 124, and t = -1.475 in t-test) than an effect summation model (p approximate to 0, df = 134, and t = 7.528 in t-test) due to the dissimilar modes of action and the complexation reactions between Cu and each of the phenolic compounds. Consequently, this study supports the importance of considering complexation reactions in assessing the combined toxicity for the formulation of water quality in mixtures of heavy metals and organic compounds, and in these cases, an independent action model was found to be appropriate. (c) 2005 Elsevier Ltd. All rights reserved.