GIST Scholar
College of Engineering
Department of Environment and Energy Engineering
1. Journal Articles
Biochemical indication of microbial mass changes using ATP and DNA measurement in biological treatment of thiocyanate
- Type
- Article
- Citation
- Applied Microbiology and Biotechnology, v.80, no.3, pp.525 - 530
- Issued Date
- 2008-09
- Abstract
- This study was designed to monitor changes in the levels of adenosine 5'-triphosphate (ATP) and deoxyribonucleic acid (DNA) per unit of microbial mass during the autotrophic biodegradation of thiocyanate (SCN(-)). An artificial medium containing trace minerals and 500 mg SCN(-)/L was used as a substrate for bacterial growth. An SCN(-)-degrading bioreactor with a working volume of 6 L, equipped with temperature, pH, and dissolved oxygen controls, was operated in batch mode. During the exponential phase of SCN(-) biodegradation, the ratios of ATP and DNA to microbial dry weight varied from 0.6 to 1.1 mu g ATP/mg of volatile suspended solid (VSS), and from 3.5 to 8.8 mu g DNA/mg of VSS, respectively. The ATP and DNA concentrations correlated linearly with microbial mass (r(2) > 0.9) within the exponential phase. The linear regression equations were as follows: (1) microbial mass concentration (mg/L) = 0.663 x ATP concentration (mu g/L) + 11.1 and (2) microbial concentration (mg/L) = 0.081 x DNA concentration (mu g/L) + 10.9. The applicable ranges were 6.8 to 47.4 mu g/L for ATP concentration and 41.5 to 395 mu g/L for DNA concentration, respectively.
- Publisher
- Springer Verlag
- ISSN
- 0175-7598
- Appears in Collections:
- Department of Environment and Energy Engineering > 1. Journal Articles
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