Effect of perfluorooctanesulfonate on osmoregulation in marine fish, Sebastes schlegeli, under different salinities

Abstract

Effect of potassium salt of perfluorooctane sulfonic acid (PFOS) on the osmoregulation of marine teleost (blackrock fish), Sebastes schlegeli, was investigated under varying salinities, by monitoring serum osmolality, Na(+), K(+), Cl(-), Ca(2+) and Mg(2+) concentrations in serum, serum glucose, and gill Na(+)-K(+) ATPase (NKA) activity. The fish was acclimatized to four salinity levels (10, 17.5, 25, and 34 psu) for 2 weeks before a 6-d exposure to 100 or 1000 mu g L(-1) of PFOS. Six fish from each exposure group were sampled at 24, 48, and 144 h after exposures, and serum, liver and gills were collected for analysis. NKA activity decreased by 34% and 31% relative to control at salinity levels of 25 and 34 psu, respectively, following exposure to 1000 mu g L(-1) PFOS. PFOS did not affect serum osmolality, Na(+), Cl(-), Ca(2+) and Mg(2+) concentrations; however, serum K(+) concentration increased during initial exposure period and then decreased after 144 h. Serum glucose levels decreased with increasing PFOS concentrations, implying high energy demand in response to exposure. Overall, PFOS exposure impaired NKA activity, altered potassium ion concentrations in serum, and reduced serum glucose levels while no other effects on serum concentrations of ionic salts were observed. (C) 2010 Elsevier Ltd. All rights reserved.