Enhanced splicing correction effect by an oligo-aspartic acid-PNA conjugate and cationic carrier complexes

Abstract

Peptide nucleic acids (PNAs) are synthetic structural analogues of DNA and RNA. They recognize specific cellular nucleic acid sequences and form stable complexes with complementary DNA or RNA. Here, we designed an oligoaspartic acid-PNA conjugate and showed its enhanced delivery into cells with high gene correction efficiency using conventional cationic carriers, such as polyethylenimine (PEI) and Lipofectamine 2000. The negatively charged oligo-aspartic acid-PNA (Asp((n))-PNA) formed complexes with PEI and Lipofectamine, and the resulting Asp((n))-PNA/PEI and Asp((n))-PNA/Lipofectamine complexes were introduced into cells. We observed significantly enhanced cellular uptake of Asp((n))-PNA by cationic carriers and detected an active splicing correction effect even at nanomolar concentrations. We found that the splicing correction efficiency of the complex depended on the kind of the cationic carriers and on the number of repeating aspartic acid units. By enhancing the cellular uptake efficiency of PNAs, these results may provide a novel platform technology of PNAs as bioactive substances for their biological and therapeutic applications. (C) 2013 Elsevier B.V. All rights reserved.