Ampoule-Like Microvolume Containers with Transparent Code for Easy-to-Use and Space-Saving Storage of Small-Volume Biospecimens

Abstract

The importance of storing, transporting, and managing biospecimens for analysis and research has increased. Biospecimens, such as DNA and cells, have a volume of several micrometers or less for practical storage, management, and usage, and high space efficiency can be achieved if stored in an appropriate indexed container. However, although previous studies have highlighted the importance of small-volume biospecimens, containers suitable for easily handling microscale samples and space-saving storage are lacking. Here, easy-to-use and space-saving biospecimen storage with ampoule-like microvolume containers and transparent codes are introduced. The high-durability epoxy-based containers are suitable for storing and using microscale biospecimens, and protecting internal biospecimens from the external environment. To identify the biospecimens, microsized transparent QR codes on microparticles for indexing sample information and storage conditions are fabricated via optofluidic maskless lithography, enabling on-demand fabrication. Both the microsized QR code and microvolume container are highly transparent, making the observation of even minimal volumes of internal material convenient. Similar to an ampoule, the microvolume container can be broken with a needle or blade to release the biospecimen. The storage efficiency of the container is verified by storing and releasing DNA, antibiotics, and cells. This container will save space for biospecimens in biobank systems.